BIO 127 : Introduction to Biotechniques
This course provides an introduction to laboratory research techniques and background as to how they are used in a variety of medical, clinical and scientific disciplines. Students will gain theoretical background and practical experience in lab safety, solid and liquid measurement, solution preparation, protein and DNA concentration determination, DNA and protein gel electrophoresis, immunoblotting, ELISA and column chromatography. Good documentation, laboratory and manufacturing practices will be applied throughout the lab. This course emphasizes basic laboratory skills essential for beginning level employment in clinical, academic, and industrial biotechnology laboratories. Pre or Corequisite: BIO 126. Two lecture hours and three laboratory hours per week. Instructional Support Fee applies. Gen. Ed. Competencies Met: Scientific Reasoning and Discovery.
Course Outcomes
1. Demonstrate appropriate chemical and biohazard safety procedures.
2. Maintain good documentation and quality control in laboratory.
3. Collect, analyze and present data from experimental procedures.
4. Demonstrate appropriate use of equipment for experimental procedures.
5. Produce and analyze recombinant DNA.
6. Maintain and manipulate both bacteria and mammalian cells in culture.
7. Isolate, separate, and analyze proteins in sequence
8. Utilize DNA and protein databases.